Acid guanidinium thiocyanatephenolchloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistryIt is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases) This method may take longer than a columnbased system such as the silicabased purification, but has higher purity and the advantage of high recovery of RNAMaterial Safety Data Sheet acc to ISO/DIS Printing date 03//13 Reviewed on 03/05/13 Trade name Guanidine hydrochloride (Contd of page 1) DR · Protective equipment Do not inhale explosion gases or combustion gases 6 Accidental release measures · Personrelated safety precautionsNot requiredGuanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation It is used as a storage buffer for whole blood samples G Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies I It is used in combination with phenol−chloroform in RNA extraction
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Guanidine thiocyanate sds-CID 35 (Guanidine) CID 781 (Thiocyanic acid) Dates Modify Create Contents 1 Structures Expand this section 2 Names and Identifiers Expand this section 3 Chemical and Physical Properties Expand this section 4 Spectral Information Expand this sectionThiocyanic acid, compound with Guanidine (11) Recommended Use Laboratory chemicals 2 HAZARDS IDENTIFICATION
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Safety Data Sheet Guanidine Hydrochloride Section 1 Identification 11 Product Identifiers Product Name Guanidine Hydrochloride CAS# RTECS# MF Synonyms Guanidine Monohydrochloride, Guanidinium Chloride, Guanidinium Hydrochloride 12 Recommended Use of the Chemical and restrictions of Use Chemical manufacturing 13 SupplierSDS Certificates ,AAAAEA,AAAABEA,AAAAIEA This product is no longer available Alternatives may be available by searching with the VWR Catalog Number listed above If you need further assistance, please call VWR Customer Service atH302 H312 H332, H314, H412 ≤100% Section 4 First Aid Measures
Stronger chaotropic agent than Guanidine Hydrochloride Used in total RNA preparation as 'guanidinium solution' Guanidine Thiocyanate is used as a common protein denaturant in the purification and extraction of mRNA, nucleic acids and proteins from cellular material, acting as a strong RNase inhibitor, protecting cellular transcripts from degradation during tissue extractionProduct Description Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria When used with acidequilibrated phenol or phenol chloroform, a solution of Guanidine Thiocyanate and betamercaptoethanolGuanidine isothiocyanate msds A member asked your answer was very unhelpful msds doesn't state any first aid recommendations was better off if i just googled it waste of time on this site ?
Description UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1) For Research Use OnlyGuanidine Isothiocyanate Solution 4 M guanidine isothiocyanate, 50 mM TrisHCl (pH 75), 25 mM EDTA Cat No Size 500 ml Store at 4°C Description Guanidine isothiocyanate is a strong protein denaturant In a highly concentrated guanidine isothiocyanate solution, many proteins unfold and separate into their constituentAC Synonyms Guanidinium isothiocyanate;
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USA US0875A USA USA US A US A US A US 0875 A US0875 A US 0875A US A US A US A US A US A US A Authority US United States Prior art keywords guanidine thiocyanate melamine ammonia guanidine thiocyanate Prior art date 1950GUANIDINE THIOCYANATE Page 1 of 6 Effective Date 05/10/17 Initial Release NONEMERGENCY TELEPHONE 24HOUR CHEMTREC EMERGENCY TELEPHONE SDS – SAFETY DATA SHEET 1 Identification Product Identifier GUANIDINE THIOCYANATE Synonyms Guanidine Isothiocyanate Chemical Formula NH2C(NH) NH2 HSCNGuanidinium thiocyanate for synthesis CAS , EC Number , chemical formula H₂NC(NH)NH₂ * HSCN Find MSDS or SDS, a COA, data sheets and more information
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To prevent DNA or RNA denaturation, the chaotropic agent, Guanadine thiocyanate is the preferred agent for use during cell lysis, and for DNA or RNA extraction This is because of its strong ability to irreversibly inhibit the activity of DNase and RNase1 day ago · This reagent extracts cytoplasmic, nuclear and 0), 5% CTAB, % SDS, 150 mM NaCl, and 10 mM EDTA), 0 4 M guanidine thiocyanate 1 M 2mercaptoethanol 0 isothiocyanate, Guanidine isothiocyanate Chemical Name CAS No Tubes were incubated at 68°C for I 09 General biochemicals, biological buffers and detergents DownloadApr 11, 01 · Guanidine samples on SDS PAGE Nick Theodorakis 4/3/01 757 AM The guanidine is likely precipitating SDS, which is not terribly soluble in many highionic strength solutions Even if it was, the high ionic strength of 6M guanide would be problematic for electrophoresis
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Description Guanidine Thiocyanate is a chaotropic agent and strong protein denaturant which inactivates nucleases approximately 25 times faster than guanidine hy SDS View Product Page17th Feb, 15 Steingrimur Stefansson Hi Kapil, urea and guanidine are more or less equal when it comes to denaturing proteins However, using urea or guanidineGuanidine thiocyanate is a water and ethanol soluble reagent used to study protein denaturation, cell signaling and function, and gene expression Guanidine thiocyanate chemical structure It is commonly used in high concentrations to extract and isolate RNA samples because it has the ability to inhibit RNase
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SDS Strong sequestering and denaturation reagent guanidine thiocyanate is also known as guanidine isothiocyanate, guandinium cyanate, guandine thiocyanate, guanidinium rhodanide, thiocyanic acid with guanidine and guanidine, monothiocyanate , with an acronym of GITC It is the essential reagent for nucleic acids sample preparation inMaterial Safety Data Sheet Guanidine Thiocyanate sc2638 Hazard Alert Code Key EXTREME HIGH MODERATE LOW Section 1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME Guanidine Thiocyanate STATEMENT OF HAZARDOUS NATURE CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR NFPA SUPPLIER SantaSynonyms Guanidine Isothiocyanate Formula NH 2 C(NH)NH 2 ·HSCN Molecular Weight g/mol CAS# EC# Component Classification Concentration Guanidine Thiocyanate Acute Tox 4;
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Safety Data Sheet for Guanidinium thiocyanate 0613 Material Safety Data Sheet or SDS for Guanidinium thiocyanate 0613 from Merck for download or viewing in the browser Catalog Number 0613 Product Name Guanidinium thiocyanate Select LanguageProcedure The following procedure is adapted from Ref 9 One milligram of tissue (or 10 6 cells) is added to 05 ml of guanidinium thiocyanate solution II and sonicated for 5 sec To this sequentially add 50 µl of 2 M sodium acetate, pH 40, 05 ml of phenol, equilibrated with water, and 01 ml of chloroform/isoamyl alcohol (24 1, v/v) withPerform RNA isolation immediately after sample collection or quickfreeze samples immediately after collection and store at –80°C or in liquid nitrogen until RNA isolation Sample type Starting material per 075 mL of TRIzol™ LS Reagent
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Guanidinium thiocyanate (GTC) or guanidinium isothiocyanate ( GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used as a nucleic acid protector in the extraction of DNA and RNA from cells GITC may also be recognized as guanidine thiocyanateView product specific information, MSDS, References and Buying FAQ read more → A useful tool in for RNA isolation and protein solubilization A strong chaotopic agent used at high concentrations for denaturing native proteins and inactivating RNAses Specifications Also known as Guanidine isothiocyanate Biotechnology Grade 80 (70%Guanidine isothiocyanate C2H5N4S CID structure, chemical names, physical and chemical properties, classification, patents, literature, biological
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The three salts that can be used are Guanidine thiocyanate, which requires ethanol Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen Ammonium acetate, which requires ethanol Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs andGuanidine Thiocyanate is inherently biodegradable Bioaccumulation is expected to be low based on inherently biodegradability a log Kow of 11 The lowest acute EC50/LC50 value from data of three trophic levels is the EC50 of 424 mg/L in invertebrates (daphnia)Guanidine and SDS solutions are denaturing solvents but we wanted, of course, to characterize the native states of membrane proteins as well The difficulty here was that there was not yet a consensus on how to define or conceive the native state – which, to newcomers like ourselves, translated to uncertainty, because we did not have the kind of experience that would enable us
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Description UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas DNA and RNA Purification and Analysis, General RNA Purification Reagents and Accessories, RNA ExtractionGuanidine Thiocyanate, Ultrapure is a freely soluble chaotropic agent routinely used for DNA/RNA extraction protocols This product is considered a dangerous good Quantities above 1 gram may be subject to additional shipping fees Please contact Customer Service for details Extraction of cellular DNA and RNA releases DNases and RNasesSynonym Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate Linear Formula NH 2 C(=NH)NH 2 · HSCN Molecular Weight
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Section 1 Chemical Product and Company Identification MSDS NameGuanidine thiocyanate Catalog NumbersAC, AC, , , BP2211, BP SynonymsThiocyanic acid, compd with guanidine (11);Apr 25, 17 · Guanidine isothiocyanate ( 1540 ) 1540 Ammonium thiocyanate ( 713 ) 713 Contact with acids or bleach liberates toxic gases DO NOT ADD acids or bleach to any liquid wastes containing this product We recommend handling all chemicals with caution SECTION 4 First aid measuresNov 01, 01 · The inclusion of SDS at the recommended concentration of 01% inhibited DNA amplification, suggesting PCR inhibition by SDS The inclusion of 003% Triton X100 and 03% Tween either alone (not shown) or together, consistently resulted in the best bacterial lysis in comparison to a mixture of NP40, SDS and Tween 5 ( Fig 1C )
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Guanidine monothiocyanate Company Identification Fisher Scientific 1 Reagent LanePopular Answers (1) I usually precipitate the samples (have to be diluted to < 2 M GuHCl first) and resuspend them in 8 M urea Then add concentrated sample buffer, heat, etc Yes, you can run 6USA USA USA USA US A US A US A US A US A US A US A US A US A US A US A US A Authority US United States Prior art keywords guanidine pressure temperature guanidine thiocyanate range Prior art date Legal
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Safety data sheet according to Regulation (EC) No 1907/06 (REACH), amended by 15/0/EU Guanidine thiocyanate ≥ 99%, for biochemistry article number 0017 Version 21 en Replaces version of 04 Version (2) date of compilation 1510Guanidine monothiocyanate, Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate CAS Catalog Number 1290 100 g 1291 500 g 1292 1 kg Storage Store at 28°C Directions forGuanidine thiocyanate (CDXG0058), CAS , is a high purity chemical used as powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids Guanidine thiocyanate is preferred for the purification of RNA
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Thiocyanic acid, compound with Guanidine (11) Recommended Use Laboratory chemicals Uses advised against Food, drug, pesticide or biocidal product use Details of the supplier of the safety data sheet Emergency Telephone Number CHEMTRECÒ, Inside the USA CHEMTRECÒ, Outside the USAChemsrc provides Guanidine thiocyanate(CAS#) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc Articles of Guanidine thiocyanate are included asSafety Data Sheet (SDS) Page 1 of 10 Date of Issue 12/29/15 6 M Guanidine Thiocyanate Buffer Section 1 Identification Product Identifier Catalog Number G4000 Recommended use of the chemical and restrictions on use For research use only Not intended for human or animal diagnostic or therapeutic uses
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Material Safety Data Sheet 1 PRODUCT AND COMPANY IDENTIFICATION Product Name Guanidine thiocyanate Cat No AC;Guanidine thiocyanate, 100 g ≥99 %, for biochemistry Write review Guanidinium isothiocyanate, GTC Working concentration 4 M in hydrous buffer system (eg 25 mM sodium citrate, 0,5 % SDS) Highly chaotropic agent that potently destroys the threedimensional structure of proteins, leading to denaturation Guanidinium (isoDr Jovita Anyanwu answered 30 years experience Internal Medicine Sorry You feel disappointed Some people may not get the magic the expect but a
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